Composite

Part:BBa_K2598018:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-09-22)


ho1+pcyA under constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1567
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1120
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The subparts are provided by Christopher A Voigt and are assembled via Gibson Assembly

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)

Levskaya, A. et al. Synthetic biology: engineering Escherichia coli to see light. Nature 438, 441-442, (2005).

Tabor, J. J. et al. A synthetic genetic edge detection program. Cell 137, 1272-1281, (2009)